Add-on Course on
BIOINFORMATICS
Objective of the Course
On completion of this course, students will have basic knowledge of the sources of sequences and protein structure data, an understanding of the relevance and importance of this data, and some exposure to basic algorithms used for processing this data.
Outline of the Course
S. No. Topic No. of Hours
1. Introduction to Genes and Proteins 2 + 2
2. Introduction to Internet Use and Search Engines 2 + 2
3. Genomic Sequence Information Sources 2 + 2
4. Protein Structure Information Sources 2 + 2
5. Introduction to Data Generating Methods 2 + 2
6. Sequence and Phylogeny Analysis 5 + 5
Lecture = 15
Practical/Tutorials = 15
Total = 30
Detailed Syllabus
1. Introduction to Genes and Proteins 4 hours
Genome Sequences
ORFs, Genes, Introns, Exons, Splice Variants
DNA/RNA Secondary Structure Triplet Coding
Protein Sequences
Protein Structure: Secondary, Tertiary, Quaternary
The notion of Homology
2. Introduction to Internet Use and Search Engines 4 hours
WWW, HTML, URLs
Browsers: Netscape / Chrome / Edge
Search Engines Google, PUBMED
3. Sequence Information Sources 4 hours
EMBL
GENBANK
Entrez
Unigene
Understanding the structure of each source and using it on the web
4. Protein Information Sources 4 hours
PDB
SwissProt
TrEMBL
Understanding the structure of each source and using it on the web
5. Introduction to Data Generating Techniques 4 hours
Restriction Enzymes, Gel Electrophoresis, Chromatograms
Blots, PCR, Microarrays, Mass Spectrometry
What data each generates, and what bioinformatic problems they pose.
6. Sequence and Phylogeny Analysis 10 hours
Detecting Open Reading Frames ….1 hours
Pairwise Alignments ….2 hours
Introduction to BLAST, using it on the web, interpreting results ….2 hours
Multiple Sequence Alignment ….3 hours
Phylogenetic Analysis ….2 hours